Nanopsy™是由先進(jìn)的分子工程納米粒子Magvigen™和Myquvigen™實(shí)現(xiàn)的，能夠有效地從蛋白質(zhì)、細(xì)胞和核酸中捕獲和識(shí)別分子信息。我們正在將nanopsy™開(kāi)發(fā)成可在成百上千家醫(yī)院部署的、自動(dòng)化和FDA批準(zhǔn)的臨床儀器和試劑盒，將致命的癌癥轉(zhuǎn)變?yōu)榭煽刂频募膊 ?/span>
用鏈霉親和素珠和生物素探針捕獲DNA
Magvigen™、Myquvigen™和Maxvigen™納米顆粒是多功能和可生物降解的磁性和/或熒光磁性納米顆粒，在加利福尼亞大學(xué)、伯克利大學(xué)、斯坦福大學(xué)和NVIGEN經(jīng)過(guò)18年的嚴(yán)格研究和開(kāi)發(fā)后達(dá)到。
| 產(chǎn)品名稱(chēng) | 應(yīng)用 | 目錄號(hào) | 規(guī)格/價(jià)格(1ml, USD) |
| MagVigen™ – Plasma DNA Capture Kit | 對(duì)血漿中的 cfDNA 的捕獲 | K61003 | Sizes (Plasma) 1,000 ml $3,999.00 |
產(chǎn)品介紹: MagVigen™ Plasma DNA Capture 磁性納米顆粒專(zhuān)為捕獲血液中的cfDNA而設(shè)計(jì)，為科研， 分子檢測(cè)工作者提供了一種簡(jiǎn)單、快速、的血液cfDNA提取方法。整個(gè)實(shí)驗(yàn)過(guò)程中無(wú)需離心或者過(guò)柱。DNA提取可以線(xiàn)性比例擴(kuò)大，輸入血漿范圍為100 uL至10mL 。分離出的cfDNA 可以用Agilent Bioanalyzer 直接定量分析。
下游應(yīng)用: PCR, DNA測(cè)序，二代測(cè)序技術(shù)(NGS), 以及測(cè)序文庫(kù)構(gòu)建。
產(chǎn)品特點(diǎn):
實(shí)驗(yàn)例子:
Figure 1. cfDNA extraction yield and size from 1.5 ml plasma samples with (blue) and without (red) spiking in DNA. Calculated yield of spiking in DNA by subtraction of endogenous cfDNA from total DNA were 80% using picogreen assay and 91% with bioanalyzer quantification.
Figure 2. cfDNA extracted from 100 µl to 5 ml of plasma was quantified by pico-green analysis.
Figure 3. cfDNA extraction yield was quantified by pico-green analysis following DNA isolation with the MagVigen™ Plasma DNA Capture Kit and kit C.
Figure 4. Beta-globin level and GAPDH levels were estimated by PCR analysis.
