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Dual-Labeled BHQ Probes 說明書

　更新時間：2017-10-30　點擊量：1621

世界*實驗材料供應商Biosearch Technologies正式授權上海起發(fā)為其中國代理，Biosearch Technologies在一直是行業(yè)的*,一直為廣大科研客戶提供zui為的產(chǎn)品和服務,上海起發(fā)一直秉承為中國科研客戶帶來的產(chǎn)品，的服務， 簽約Biosearch Technologies就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務,您的滿意將是我們zui大的收獲

Biosearch Technologies中國代理，Biosearch Technologies上海代理，Biosearch Technologies北京代理，Biosearch Technologies廣東代理，Biosearch Technologies江蘇代理 Biosearch Technologies湖北代理, Biosearch Technologies天津, Biosearch Technologies黑龍江代理, Biosearch Technologies內(nèi)蒙古代理, Biosearch Technologies吉林代理, Biosearch Technologies福建代理, Biosearch Technologies江蘇代理, Biosearch Technologies浙江代理, Biosearch Technologies四川代理,

 

Biosearch Technologies是一家致力于創(chuàng)新性研發(fā)新型核酸產(chǎn)品以促進新基因的發(fā)現(xiàn)與應用的高科技公司。從上世紀八十年代開始,一代一代的Biosearch創(chuàng)新者就專注于寡核苷酸化學合成技術， 在設計和生產(chǎn)熒光探針及引物中經(jīng)過多次改良形成了公司*的寡核苷酸修飾技術。目前 Biosearch開發(fā)的熒光粹滅技術Black Hole Quencher® (BHQ® dyes) 已成為熒光探針設計/合成的工業(yè)生產(chǎn)標準。以創(chuàng)新研發(fā)為原發(fā)動力的Biosearch Technologies公司已經(jīng)通過英國ISO 9001:2008、ISO 13485:2003標準認證，獲得加利福尼亞設備制造許可證以及GMP生產(chǎn)許可證。Biosearch擁有豐富的高品質(zhì)產(chǎn)品線，不僅包括探針和引物產(chǎn)品，廣泛應用于藥物研發(fā)，DNA測序/SNP檢測/基因表達分析等領域，也為其他寡核苷酸生產(chǎn)廠商提供原材料。公司在儀器生產(chǎn)領域的努力也使得我們能對公司產(chǎn)品的合成和純化進一步深入和發(fā)展。

 

Dual-Labeled BHQ® Probes

雙標簽BHQ®探頭

 

產(chǎn)品信息

img07.jpg

雙標簽BHQ探針是傳統(tǒng)的線性FRET探針，其包含熒光團和猝滅劑，其共價連接到通常具有20至30個堿基的寡核苷酸的5'和3'末端。熒光通過Taq聚合酶的5'核酸外切酶活性釋放，Taq聚合酶在探針與其互補序列雜交時切斷熒光染料。雙標簽BHQ探針是檢測特定目標序列量的存在和量化的理想選擇。

我們還提供ValuMix用于基因表達和qPCR，其在單個管中提供一個BHQ探針和兩個引物。可以圍繞遞送的0.5nmol，5nmol或20nmol的探針量靈活地調(diào)節(jié)Valumix引物量。根據(jù)您的偏好，探針到底漆比可以在1：1至1：4.5之間。ValuMix是一種經(jīng)濟的選擇，提供0.5nmol的FAM-BHQ探針，僅用$ 79混合引物。有關各種選項，請參閱產(chǎn)品列表選項卡。

設計關注BHQ探針

請注意，長度超過30個堿基的序列不可能作為末端標記的探針進行有效的猝滅。對于這種情況，請考慮在內(nèi)部定位淬火劑。相反，短于20個堿基的序列可能被設計為次要粘結劑（MGB™）探針，并且不太可能在70℃的適當溫度下結合。請考慮訂購BHQplus®探針的順序，否則請techsupport@biosearchtech.com與探針設計有任何疑問。

注意：

  • 使用內(nèi)部淬火器構建探頭（目前僅適用于FAM）
  • 添加由T（...）注釋的內(nèi)部修飾表示胸苷堿基附加到內(nèi)部修飾。請注意，添加此類內(nèi)部修改時，將在序列中引入額外的T基。
  • 建筑探頭與搖擺/退化基地（什么是擺動？）
  • 為了確保對寡核苷酸進行zui有效的處理，在序列輸入字段中輸入擺動/退化堿基時使用IUPAC代碼，而不是使用括號（例如（A / G），（A / G / C / T）等）

 

?部分相關產(chǎn)品如下：

貨號品名NOTE
DLO-FB1-1Dual-labeled Probe, 5' FAM/3' BHQ-1Provides > 60 nmol pure.
DLO-FB1-2Dual-labeled Probe, 5' FAM/3' BHQ-1Provides > 25 nmol pure.
DLO-FB1-5Dual-labeled Probe, 5' FAM/3' BHQ-1Provides > 10 nmol pure.
DLO-FT-1Dual-labeled Probe, 5' FAM/3' TAMRAProvides > 60 nmol pure.
DLO-FT-2Dual-labeled Probe, 5' FAM/3' TAMRAProvides > 25 nmol pure.
DLO-FT-5Dual-labeled Probe, 5' FAM/3' TAMRAProvides > 10 nmol pure.
DLO-HB1-1Dual-labeled Probe, 5' HEX/3' BHQ-1Provides > 60 nmol pure.
DLO-HB1-2Dual-labeled Probe, 5' HEX/3' BHQ-1Provides > 25 nmol pure.
DLO-HB1-5Dual-labeled Probe, 5' HEX/3' BHQ-1Provides > 10 nmol pure.
DLO-JB1-1Dual-labeled Probe, 5' JOE/3' BHQ-1Provides > 30 nmol pure.
DLO-JB1-2Dual-labeled Probe, 5' JOE/3' BHQ-1Provides > 10 nmol pure.
DLO-RB2-1Dual-labeled Probe, 5' ROX/3' BHQ-2Provides > 30 nmol pure.
DLO-RB2-2Dual-labeled Probe, 5' ROX/3' BHQ-2Provides > 10 nmol pure.
DLO-TEB1-1Dual-labeled Probe, 5' TET/3' BHQ-1Provides > 60 nmol pure.
DLO-TEB1-2Dual-labeled Probe, 5' TET/3' BHQ-1Provides > 25 nmol pure.
DLO-TEB1-5Dual-labeled Probe, 5' TET/3' BHQ-1Provides > 10 nmol pure.
DLO-TB2-1Dual-labeled Probe, 5' TAMRA/3' BHQ-2Provides > 60 nmol pure.
DLO-TB2-2Dual-labeled Probe, 5' TAMRA/3' BHQ-2Provides > 25 nmol pure.
DLO-TB2-5Dual-labeled Probe, 5' TAMRA/3' BHQ-2Provides > 10 nmol pure.
DLO-TET-1Dual-labeled Probe, 5' TET/3' TAMRAProvides > 60 nmol pure.
DLO-TET-2Dual-labeled Probe, 5' TET/3' TAMRAProvides > 25 nmol pure.
DLO-TET-5Dual-labeled Probe, 5' TET/3' TAMRAProvides > 10 nmol pure.
DLO-CAB2-2Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2Provides > 25 nmol pure.
DLO-CAB2-5Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2Provides > 10 nmol pure.
DLO-CAB2-1Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2Provides > 60 nmol pure.
DLO-Q6B2-5Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2Provides 10 nmol pure.
DLO-Q6B2-2Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2Provides > 20 nmol pure.
DLO-Q6B2-1Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2Provides > 40 nmol pure.
DLO-Q5B2-5Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2Provides > 10 nmol pure.
DLO-Q5B2-2Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2Provides > 25 nmol pure.
DLO-Q5B2-1Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2Provides > 60 nmol pure.
DLO-Q6B3-2Dual-labeled Probe, 5' Quasar 670/3’ BHQ-3Provides > 20 nmol pure.
DLO-Q6B3-1Dual-labeled Probe, 5' Quasar 670/3’ BHQ-3Provides > 40 nmol pure.
DLO-COB1-1Dual-labeled Probe, 5' CAL Fluor® Orange 560/3' BHQ-1Provides > 60 nmol pure.
DLO-COB1-2Dual-labeled Probe, 5' CAL Fluor® Orange 560/3' BHQ-1Provides > 25 nmol pure.
DLO-COB1-5Dual-labeled Probe, 5' CAL Fluor® Orange 560/3' BHQ-1Provides > 10 nmol pure.
DLO-B2P6-2Dual-labeled Probe, 5’ BHQ-2/3’ Pulsar 650Provides > 10 nmol pure.
DLO-B2P6-1Dual-labeled Probe, 5’ BHQ-2/3’ Pulsar 650Provides > 40 nmol pure.
DLO-CGB1-5Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1Provides > 10 nmol pure.
DLO-CGB1-2Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1Provides > 25 nmol pure.
DLO-CGB1-1Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1Provides > 60 nmol pure.
DLO-C635B2-5Dual-labeled Probe, 5' CAL Fluor® Red 635/3' BHQ-2Provides > 3 nmol pure.
DLO-C590B2-5Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2Provides > 10 nmol pure.
DLO-F(T-B1)-2Dual-labeled Probe, 5' FAM/Internal T-BHQ-1/3’ C3Provides > 10 nmol pure.
DLO-F(T-B1)-1Dual-labeled Probe, 5' FAM/Internal T-BHQ-1/3’ C3Provides > 30 nmol pure.
DLO-C590B2-2Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2Provides > 25 nmol pure.
DLO-C590B2-1Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2Provides > 60 nmol pure.
DLO-C635B2-2Dual-labeled Probe, 5' CAL Fluor® Red 635/3' BHQ-2Provides > 10 nmol pure.
DLO-C635B2-1Dual-labeled Probe, 5' CAL Fluor® Red 635/3' BHQ-2Provides > 40 nmol pure.
DLO-RFB-5ValuProbe™, 5' FAM/3' BHQ-1Provides 10 nmol pure.
DLO-Q7B2-5Dual-labeled Probe, 5' Quasar 705/3’ BHQ-2Provides > 3 nmol pure.
DLO-Q7B2-2Dual-labeled Probe, 5' Quasar 705/3’ BHQ-2Provides > 10 nmol pure.
DLO-Q7B2-1Dual-labeled Probe, 5' Quasar 705/3’ BHQ-2Provides > 40 nmol pure.
DLO-FT-25Dual-labeled Probe, 5' FAM/3' TAMRAProvides 5 nmol pure.
DLO-TEB1-25Dual-labeled Probe, 5' TET/3' BHQ-1Provides 5 nmol pure.
DLO-TET-25Dual-labeled Probe, 5' TET/3' TAMRAProvides 5 nmol pure.
DLO-HB1-25Dual-labeled Probe, 5' HEX/3' BHQ-1Provides 5 nmol pure.
DLO-Q6B2-25Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2Provides 5 nmol pure.
DLO-Q5B2-25Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2Provides 5 nmol pure.
DLO-CAB2-25Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2Provides 5 nmol pure.
DLO-COB1-25Dual-labeled Probe, 5' CAL Fluor® Orange 560/3' BHQ-1Provides 5 nmol pure.
DLO-CGB1-25Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1Provides 5 nmol pure.
DLO-C590B2-25Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2Provides 5 nmol pure.
DLO-TB2-25Dual-labeled Probe, 5' TAMRA/3' BHQ-2Provides 5 nmol pure
DLO-Q7B3-2Dual-labeled Probe, 5' Quasar 705/3’ BHQ-3Provides > 10 nmol pure.
DLO-Q7B3-1Dual-labeled Probe, 5' Quasar 705/3’ BHQ-3Provides > 40 nmol pure.
DLO-Q6B3-5Dual-labeled Probe, 5' Quasar 670/3’ BHQ-3Provides 10 nmol pure.
DLO-Q6B3-25Dual-labeled Probe, 5' Quasar 670/3’ BHQ-3Provides 5 nmol pure.
DLO-Q7B3-5Dual-labeled Probe, 5' Quasar 705/3’ BHQ-3Provides > 3 nmol pure.
DLOM-RFB-05FAM Dual-labeled BHQ-1 Probe with Primers, Mixed0.5 nmol
DLOM-RFB-5FAM Dual-labeled BHQ-1 Probe with Primers, Mixed5 nmol
DLOM-RFB-20FAM Dual-labeled BHQ-1 Probe with Primers, Mixed20 nmol
DLOM-COB1-5CAL Fluor Orange 560 Dual-labeled BHQ-1 Probe with Primers, Mixed5 nmol
DLOM-COB1-20CAL Fluor Orange 560 Dual-labeled BHQ-1 Probe with Primers, Mixed20 nmol
DLOM-CAB2-5CAL Fluor Red 610 Dual-labeled BHQ-2 Probe with Primers, Mixed5 nmol
DLOM-CAB2-20CAL Fluor Red 610 Dual-labeled BHQ-2 Probe with Primers, Mixed20 nmol
DLOM-Q6B2-5Quasar 670 Dual-labeled BHQ-2 Probe with Primers, Mixed5 nmol
DLOM-Q6B2-20Quasar 670 Dual-labeled BHQ-2 Probe with Primers, Mixed20 nmol
DLOM-F(T-B1)-5FAM Dual-labeled Internal T-BHQ-1 Probe with Primers, Mixed5 nmol
DLOM-F(T-B1)-20FAM Dual-labeled Internal T-BHQ-1 Probe with Primers, Mixed20 nmol

 

我們公司zui大優(yōu)勢是強大的采購，

1：基本什么都能進口，血清，抗體，耗材，還有部分限制進口的，

2：貨品全，現(xiàn)經(jīng)營過700多個品牌，基本所有生物試劑耗材都可以進口，特別是冷偏的產(chǎn)品那就更有優(yōu)勢，

3：提供加急服務，一般1-2周到貨，超過時限加急費全免

4：價格公道，絕大部分價格有優(yōu)勢，當然不能保證100%產(chǎn)品都是,因為意味著沒有服務.

5：良好的信譽，大部分客戶我們提供貨到付款服務，客戶包括清華，北大 交大 復旦，中山等100多所大學，ROCHE，阿斯利康，國藥 ，fisher等500多家公司

6：我們*代理的品牌有：Antibody Research Corporation，arcticzymes，Biorelevant，AmberGen, Inc. ，clemente-associates，clodronaiposomes，Columbia Biosciences，enzyme research，Gene Bridges GmbH，Genovis，AmberGen, Inc.  Biotechnology GmbH，Haematologic Technologies HTI Haemtech，hookelabs，Immudex，Innovative Research of America，inspiralis ，List Biological Laboratories, Inc.，lumafluor，Microsurfaces，multiplicom，nanotools，Pel-Freez Biologicals，pentapharm，progen，Protein Ark，QA-Bio,Inc，QA-Bio,IncQuickZyme Biosciences，Teknova，TriLink BioTechnologies, Inc.，Zyagen Laboratories 等

7：我們還是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批發(fā)，歡迎合作。